Matrices listed below were spiked with certain level of PROX1 and the recovery rates were calculated by comparing the measured value to the expected amount of PROX1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
96
EDTA plasma(n=5)
89-102
97
heparin plasma(n=5)
89-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PROX1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-103%
88-93%
95-105%
EDTA plasma(n=5)
83-97%
83-96%
83-97%
heparin plasma(n=5)
88-98%
89-98%
84-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q92786
Applications
ELISA
Applications Notes
PROX1 ELISA Kit allows for the in vitro quantitative determination of PROX1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human PROX1 (Prospero homeobox protein 1)ELISA Kit