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Home / ELISA Kit

Human Protocadherin beta-15 (PCDHB15) ELISA Kit

SKU : BHE13703513

Protocadherin beta-15 is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins.

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Mfr.No.	KTE61297
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protocadherin beta-15 (PCDHB15) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHB15.
Alternative Names	PCDHB15; PCDH-BETA15;
Detection Method	Colorimetric
Kit Components	Human Protocadherin beta-15 microplate Human Protocadherin beta-15 standard Human Protocadherin beta-15 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protocadherin beta-15 (PCDHB15) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCDHB15. No significant cross-reactivity or interference between Human PCDHB15 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protocadherin beta-15 is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins.
Notes	This Human Protocadherin beta-15 (PCDHB15) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHB15 in samples. An antibody specific for PCDHB15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHB15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHB15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHB15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56121

Applications	ELISA
Applications Notes	This Human Protocadherin beta-15 (PCDHB15) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHB15 in samples. An antibody specific for PCDHB15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHB15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHB15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHB15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703513	KTE61297	48 T	$339		Add to Cart
BHE13703513	KTE61297	96 T	$589		Add to Cart
BHE13703513	KTE61297	96 Tx5	$2629		Add to Cart
BHE13703513	KTE61297	96 Tx50	$24569		Add to Cart

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