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Home / ELISA Kit

Human Prothymosin, alpha (PTMA) ELISA Kit

SKU : BHE13703275

Prothymosin alpha is a protein encoded by the PTMA. PTMA is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin alpha-1. The encoded human PTMA protein is a highly acidic (54 residues out of 111) and shared over 90% sequence homology with rat PTMA. The primary structure of PTMA is highly conserved and shows a number of important features. The first 28 amino acids of its sequence correspond to those of thymosin-alpha-1. It has a central acidic region (residues 41-85) comprising of glutamic and aspartic residues. Neither type of secondary structure has been detected. Thymosin-alpha-1 appeared at positions 2-29 of the PTMA.PTMA has been shown to serve essential biological functions.

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Mfr.No.	KTE61059
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prothymosin, alpha (PTMA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTMA.
Alternative Names	PTMA; MGC104802; TMSA; gene sequence 28; prothymosin alpha protein
Detection Method	Colorimetric
Kit Components	Human Prothymosin, alpha microplate Human Prothymosin, alpha standard Human Prothymosin, alpha detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prothymosin, alpha (PTMA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTMA. No significant cross-reactivity or interference between Human PTMA and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prothymosin alpha is a protein encoded by the PTMA. PTMA is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin alpha-1. The encoded human PTMA protein is a highly acidic (54 residues out of 111) and shared over 90% sequence homology with rat PTMA. The primary structure of PTMA is highly conserved and shows a number of important features. The first 28 amino acids of its sequence correspond to those of thymosin-alpha-1. It has a central acidic region (residues 41-85) comprising of glutamic and aspartic residues. Neither type of secondary structure has been detected. Thymosin-alpha-1 appeared at positions 2-29 of the PTMA.PTMA has been shown to serve essential biological functions.
Notes	This Human Prothymosin, alpha (PTMA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTMA in samples. An antibody specific for PTMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Prothymosin, alpha (PTMA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTMA in samples. An antibody specific for PTMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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