Human Proteolysis Inducing Factor/Dermcidin(PIF/DCD)ELISA Kit

Mfr.No.

CSB-E13626h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

dermcidin, AIDD, DCD-1, DSEP, HCAP, MGC71930, PIF, diffusible survival/evasion peptide, preproteolysin, proteolysis inducing factor, survival promoting peptide

Recovery

The recovery of human F? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-99
EDTA plasma (n=4)	95	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human F? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	84-99
1:2000	Average %	95
	Range %	91-100
1:4000	Average %	88
	Range %	86-98
1:8000	Average %	95
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.711	2.684	2.698	2.614
20	2.084	2.106	2.095	2.011
10	1.396	1.415	1.406	1.322
5	0.778	0.758	0.768	0.684
2.5	0.499	0.505	0.502	0.418
1.25	0.295	0.293	0.294	0.210
0.625	0.176	0.182	0.179	0.095
0	0.082	0.085	0.084	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P81605

Applications	ELISA
Research Area	DCD

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504051	CSB-E13626h	96T	$790	Add to Cart
BHE10504051	CSB-E13626h	5x96T	$3792	Add to Cart
BHE10504051	CSB-E13626h	10x96T	$7505	Add to Cart

Human Proteolysis Inducing Factor/Dermcidin(PIF/DCD)ELISA Kit

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