Human Protein Wnt-7B - WNT7B ELISA Kit

Mfr.No.

E6014Hu

Sensitivity

0.022ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Protein Wnt-7b;WNT7B

Molecular Weight

39327

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6014Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.83	0.23	5.91
Intra-Assay	2	18	7.65	0.26	3.33
Intra-Assay	3	18	2.23	0.14	6.42

Background

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314).

Protocol

These standard curves of E6014Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.13	2.207	2.153
	2.284
6ng/mL	1.285	1.308	1.254
	1.332
3ng/mL	0.847	0.785	0.731
	0.723
1.5ng/mL	0.552	0.537	0.483
	0.522
0.75ng/mL	0.288	0.291	0.237
	0.294
0ng/mL	0.07	0.054	0
	0.038

Gene ID	7477
UniProt ID	P56706

Research Area	Stem Cells; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107376	E6014Hu	48T	$320		Add to Cart
BHE12107376	E6014Hu	96T	$458		Add to Cart

Human Protein Wnt-7B - WNT7B ELISA Kit

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