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Home / ELISA Kit

Human Protein tyrosine phosphatase type IVA 2 (PTP4A2) ELISA Kit

SKU : BHE13703272

Protein tyrosine phosphatase type IVA 2 is an enzyme belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis.

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Mfr.No.	KTE61056
Description	This Human Protein tyrosine phosphatase type IVA 2 (PTP4A2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTP4A2.
Alternative Names	PTP4A2; HH13; HH7-2; HU-PP-1; OV-1; PRL-2; PRL2; PTP4A; PTPCAAX2; ptp-IV1a; ptp-IV1b; phosphatase of regenerating liver 2; protein tyrosine phosphatase IVA; protein tyrosine phosphatase IVA2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Protein tyrosine phosphatase type IVA 2 microplate Human Protein tyrosine phosphatase type IVA 2 standard Human Protein tyrosine phosphatase type IVA 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein tyrosine phosphatase type IVA 2 (PTP4A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTP4A2. No significant cross-reactivity or interference between Human PTP4A2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protein tyrosine phosphatase type IVA 2 is an enzyme belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis.
Notes	This Human Protein tyrosine phosphatase type IVA 2 (PTP4A2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTP4A2 in samples. An antibody specific for PTP4A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTP4A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTP4A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTP4A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	8073
Antigen	PTP4A2
Tag	PTP4A2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Protein tyrosine phosphatase type IVA 2 (PTP4A2) ELISA Kit employs a two-site sandwich ELISA to quantitate PTP4A2 in samples. An antibody specific for PTP4A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTP4A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTP4A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTP4A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703272	KTE61056	48 T	$339		Add to Cart
BHE13703272	KTE61056	96 T	$589		Add to Cart
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BHE13703272	KTE61056	96 Tx50	$24569		Add to Cart

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