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Home / ELISA Kit

Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit

SKU : BHE13703277

Protein tyrosine kinase 2 beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2.

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Mfr.No.	KTE61061
Description	This Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2B.
Alternative Names	PTK2B; CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK; CAK beta; calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesion kinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Protein-tyrosine kinase 2-beta microplate Human Protein-tyrosine kinase 2-beta standard Human Protein-tyrosine kinase 2-beta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTK2B. No significant cross-reactivity or interference between Human PTK2B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protein tyrosine kinase 2 beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2.
Notes	This Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2B in samples. An antibody specific for PTK2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	2185
Antigen	PTK2B
Tag	PTK2B
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit employs a two-site sandwich ELISA to quantitate PTK2B in samples. An antibody specific for PTK2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTK2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703277	KTE61061	48 T	$339		Add to Cart
BHE13703277	KTE61061	96 T	$589		Add to Cart
BHE13703277	KTE61061	96 Tx5	$2629		Add to Cart
BHE13703277	KTE61061	96 Tx50	$24569		Add to Cart

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