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Home / ELISA Kit

Human Protein SSX2 (SSX2) ELISA Kit

SKU : BHE13702589

SSX2 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.

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Mfr.No.	KTE60372
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2.
Alternative Names	SSX2; RP11-552J9.2; CT5.2a; HD21; HOM-MEL-40; MGC119055; MGC15364; MGC3884; SSX; cancer/testis antigen family 5; member 2a; sarcoma; synovial; X-chromosome-related 2; synovial sarcoma; X breakpoint 2; isofo
Detection Method	Colorimetric
Kit Components	Human Protein SSX2 microplate Human Protein SSX2 standard Human Protein SSX2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein SSX2 (SSX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSX2. No significant cross-reactivity or interference between Human SSX2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SSX2 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
Notes	This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2 in samples. An antibody specific for SSX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6757

Applications	ELISA
Applications Notes	This Human Protein SSX2 (SSX2) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX2 in samples. An antibody specific for SSX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702589	KTE60372	48 T	$339		Add to Cart
BHE13702589	KTE60372	96 T	$589		Add to Cart
BHE13702589	KTE60372	96 Tx5	$2629		Add to Cart
BHE13702589	KTE60372	96 Tx50	$24569		Add to Cart

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