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Home / ELISA Kit

Human Protein SPHAR (SPHAR) ELISA Kit

SKU : BHE13702746

Protein SPHAR (SPHAR) also named S-phase response protein , which contains 63 amino acids with 7515 Da. We still know little about this gene now , Please reaserch hard !

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE60530
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein SPHAR (SPHAR) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHAR.
Alternative Names	SPHAR; MGC88280; RP4-803J11.4; S-phase response (cyclin-related)
Detection Method	Colorimetric
Kit Components	Human Protein SPHAR microplate Human Protein SPHAR standard Human Protein SPHAR detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein SPHAR (SPHAR) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPHAR. No significant cross-reactivity or interference between Human SPHAR and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protein SPHAR (SPHAR) also named S-phase response protein , which contains 63 amino acids with 7515 Da. We still know little about this gene now , Please reaserch hard !
Notes	This Human Protein SPHAR (SPHAR) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHAR in samples. An antibody specific for SPHAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPHAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHAR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10638

Applications	ELISA
Applications Notes	This Human Protein SPHAR (SPHAR) ELISA Kit employs a two-site sandwich ELISA to quantitate SPHAR in samples. An antibody specific for SPHAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPHAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPHAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPHAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702746	KTE60530	48 T	$339		Add to Cart
BHE13702746	KTE60530	96 T	$589		Add to Cart
BHE13702746	KTE60530	96 Tx5	$2629		Add to Cart
BHE13702746	KTE60530	96 Tx50	$24569		Add to Cart

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