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Home / ELISA Kit

Human Protein sidekick-1 (SDK1) ELISA Kit

SKU : BHE13702941

The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.

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Mfr.No.	KTE60725
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein sidekick-1 (SDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SDK1.
Alternative Names	SDK1; FLJ31425; sidekick 1
Detection Method	Colorimetric
Kit Components	Human Protein sidekick-1 microplate Human Protein sidekick-1 standard Human Protein sidekick-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein sidekick-1 (SDK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDK1. No significant cross-reactivity or interference between Human SDK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.
Notes	This Human Protein sidekick-1 (SDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SDK1 in samples. An antibody specific for SDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	221935

Applications	ELISA
Applications Notes	This Human Protein sidekick-1 (SDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SDK1 in samples. An antibody specific for SDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702941	KTE60725	48 T	$339		Add to Cart
BHE13702941	KTE60725	96 T	$589		Add to Cart
BHE13702941	KTE60725	96 Tx5	$2629		Add to Cart
BHE13702941	KTE60725	96 Tx50	$24569		Add to Cart

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