Human Protein S100-A7A(S100A7A) ELISA kit

Human Protein S100-A7A(S100A7A) ELISA kit

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Mfr.No.

CSB-EL020636HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

S100 calcium binding protein A7A, NICE-2, S100A15, S100A7L1, S100A7f, OTTHUMP00000015325, S100 calcium binding protein A15, S100 calcium binding protein A7-like 1

Recovery

The recovery of human IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	89	85-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	103
	Range %	99-108
1:4	Average %	80
	Range %	85-96
1:8	Average %	93
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.850	1.920	1.885	1.795
400	1.342	1.405	1.374	1.284
200	0.834	0.902	0.868	0.778
100	0.557	0.525	0.541	0.451
50	0.325	0.342	0.334	0.244
25	0.253	0.264	0.259	0.169
12.5	0.146	0.150	0.148	0.058
0	0.087	0.092	0.090	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q86SG5

Applications	ELISA
Research Area	S100A7A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504020	CSB-EL020636HU	96T	$695	Add to Cart
BHE10504020	CSB-EL020636HU	5x96T	$3336	Add to Cart
BHE10504020	CSB-EL020636HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.