Human Protein S100-A4(S100A4) ELISA kit

Human Protein S100-A4(S100A4) ELISA kit

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Mfr.No.

CSB-EL020632HU

Alternative Names

S100 calcium binding protein A4, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, OTTHUMP00000015469, OTTHUMP00000032895, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-bind

Reactivity

Human

Detection Range

0.9 ng/mL-60 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-98
EDTA plasma (n=4)	93	88-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-95
1:2	Average %	85
	Range %	80-95
1:4	Average %	90
	Range %	86-100
1:8	Average %	92
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	1.865	1.802	1.834	1.739
3000	1.418	1.376	1.397	1.302
1500	1.036	1.004	1.020	0.925
750	0.624	0.589	0.607	0.512
375	0.379	0.364	0.372	0.277
187.5	0.211	0.209	0.210	0.115
93.75	0.132	0.122	0.127	0.032
0	0.099	0.091	0.095	?

Species

Homo sapiens

Sensitivity

0.225 ng/mL

Limit Of Detection

450 nm

UniProt ID	P26447
Antigen	S100 Calcium Binding Protein A4

Applications

ELISA

Research Area

S100A4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504018	CSB-EL020632HU	96T	$695	Add to Cart
BHE10504018	CSB-EL020632HU	5x96T	$3336	Add to Cart
BHE10504018	CSB-EL020632HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.