Human protein Red (IK) ELISA kit

Human protein Red (IK) ELISA kit

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Mfr.No.

CSB-EL011570HU

Alternative Names

protein Red, Cytokine IK_x000D_ IK factor_x000D_ Protein RER _x000D_ IK_x000D_ RED, RER_x000D_, protein Red, Cytokine IK_x000D_ IK factor_x000D_ Protein RER _x000D_ IK_x000D_ RED, RER_x000D_

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-99
EDTA plasma (n=4)	97	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-91
1:2	Average %	98
	Range %	91-104
1:4	Average %	97
	Range %	90-105
1:8	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	1.756	1.737	1.747	1.601
250	1.134	1.142	1.138	0.992
125	0.702	0.719	0.711	0.565
62.5	0.465	0.450	0.458	0.312
31.2	0.337	0.349	0.343	0.197
15.6	0.266	0.272	0.269	0.123
7.8	0.192	0.203	0.198	0.052
0	0.145	0.147	0.146	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q13123
Antigen	protein Red (IK)

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504013	CSB-EL011570HU	96T	$695	Add to Cart
BHE10504013	CSB-EL011570HU	5x96T	$3336	Add to Cart
BHE10504013	CSB-EL011570HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.