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Home / ELISA Kit

Human Protein quaking (QKI) ELISA Kit

SKU : BHE13703222

QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1.
The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.The balance between the nuclear and cytoplasmic isoforms of Qk1 controlled the nuclear export of Mbp mRNAs and the cellular localization of the 17- and 21.5-kD Mbp isoforms. Overexpression of Qki-5 in cultured rodent oligodendrocytes recreated the Mbp defects observed in qk(v) mice, with retention of Mbp in the nucleus.

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Mfr.No.	KTE61006
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein quaking (QKI) ELISA Kit employs a two-site sandwich ELISA to quantitate QKI.
Alternative Names	QKI; DKFZp586I0923; Hqk; QK; QK1; QK3; RNA binding protein HQK; homolog of mouse quaking QKI (KH domain RNA binding protein); quaking homolog; KH domain RNA binding
Detection Method	Colorimetric
Kit Components	Human Protein quaking microplate Human Protein quaking standard Human Protein quaking detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein quaking (QKI) ELISA Kit has high sensitivity and excellent specificity for detection of Human QKI. No significant cross-reactivity or interference between Human QKI and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.The balance between the nuclear and cytoplasmic isoforms of Qk1 controlled the nuclear export of Mbp mRNAs and the cellular localization of the 17- and 21.5-kD Mbp isoforms. Overexpression of Qki-5 in cultured rodent oligodendrocytes recreated the Mbp defects observed in qk(v) mice, with retention of Mbp in the nucleus.
Notes	This Human Protein quaking (QKI) ELISA Kit employs a two-site sandwich ELISA to quantitate QKI in samples. An antibody specific for QKI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQKI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QKI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QKI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9444

Applications	ELISA
Applications Notes	This Human Protein quaking (QKI) ELISA Kit employs a two-site sandwich ELISA to quantitate QKI in samples. An antibody specific for QKI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQKI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QKI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QKI bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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