Human Protein prune homolog(PRUNE) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL018831HU

Alternative Names

prune homolog (Drosophila), DRES-17, DRES17, HTCD37, Drosophila-related expressed sequence 17, prune

Reactivity

Human

Detection Range

47 pg/mL-3000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of chicken IL-1? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	99
	Range %	95-106
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

11.75 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q86TP1
Antigen	prune homolog (Drosophila)

Applications

ELISA

Research Area

PRUNE

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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