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Home / ELISA Kit

Human Protein phosphatase Slingshot homolog 3 (SSH3) ELISA Kit

SKU : BHE13702585

The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo.
Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L, SSH1S, and SSH1B, and SSH2 encodes 2 isoforms, SSH2 and SSH2B. The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs (domains A and B) and are unique to the SSH family.

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Mfr.No.	KTE60368
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein phosphatase Slingshot homolog 3 (SSH3) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH3.
Alternative Names	SSH3; FLJ10928; FLJ20515; FLJ42240; SSH3L; slingshot 3; slingshot homolog 3
Detection Method	Colorimetric
Kit Components	Human Protein phosphatase Slingshot homolog 3 microplate Human Protein phosphatase Slingshot homolog 3 standard Human Protein phosphatase Slingshot homolog 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein phosphatase Slingshot homolog 3 (SSH3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSH3. No significant cross-reactivity or interference between Human SSH3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo. Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L, SSH1S, and SSH1B, and SSH2 encodes 2 isoforms, SSH2 and SSH2B. The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs (domains A and B) and are unique to the SSH family.
Notes	This Human Protein phosphatase Slingshot homolog 3 (SSH3) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH3 in samples. An antibody specific for SSH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	54961

Applications	ELISA
Applications Notes	This Human Protein phosphatase Slingshot homolog 3 (SSH3) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH3 in samples. An antibody specific for SSH3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSH3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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