Human Protein Phosphatase 2A

Mfr.No.

E6870Hu

Sensitivity

0.018ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PP2C-alpha;PPM 1A;PPM1A;Protein phosphatase 1A;Protein phosphatase 2C isoform alpha;Protein phosphatase IA

Molecular Weight

42448

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6870Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.1	3.2
Intra-Assay	2	18	1.6	0.1	6.2
Intra-Assay	3	18	1.3	0.08	6.6

Background

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

Protocol

These standard curves of E6870Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.57	2.556	2.519
	2.541
3.2ng/ml	1.482	1.417	1.38
	1.351
1.6ng/ml	0.936	0.873	0.836
	0.809
0.8ng/ml	0.427	0.473	0.436
	0.518
0.4ng/ml	0.271	0.234	0.197
	0.197
0ng/ml	0.028	0.037	0
	0.045

Gene ID	5494
UniProt ID	P35813

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115569	E6870Hu	48T	$320		Add to Cart
BHE12115569	E6870Hu	96T	$458		Add to Cart

Human Protein Phosphatase 2A - PP2A ELISA Kit

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