Human Protein Phosphatase 1B

Mfr.No.

E3842Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PP2C-beta;PPM 1B;PPM1B;Protein phosphatase 1B;Protein phosphatase 2C isoform beta

Molecular Weight

52643

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3842Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.2	0.18	4.3
Intra-Assay	2	18	2	0.09	4.3
Intra-Assay	3	18	1	0.05	4.8

Background

Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

Protocol

These standard curves of E3842Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.243	2.254	2.21
	2.266
4ng/ml	1.479	1.472	1.427
	1.464
2ng/ml	0.903	0.911	0.867
	0.92
1ng/ml	0.424	0.46	0.415
	0.496
0.5ng/ml	0.247	0.248	0.204
	0.249
0ng/ml	0.054	0.045	0
	0.035

Gene ID	5495
UniProt ID	O75688

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105287	E3842Hu	48T	$320		Add to Cart
BHE12105287	E3842Hu	96T	$458		Add to Cart

Human Protein Phosphatase 1B - PPM1B ELISA Kit

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