Human protein(peptidylprolyl cis/trans isomerase)NIMA-interacting 1,PIN1 ELISA Kit

Human protein(peptidylprolyl cis/trans isomerase)NIMA-interacting 1,PIN1 ELISA Kit

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Mfr.No.

CSB-E11308h

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peptidylprolyl cis/trans isomerase, NIMA-interacting 1, DOD, UBL5, peptidyl-prolyl cis/trans isomerase, NIMA-interacting, prolyl isomerase, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, protein (peptidylprolyl cis/trans isomerase) NIMA-i

Recovery

The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-109
1:2	Average %	97
	Range %	91-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.675	2.552	2.614	2.439
400	2.065	2.076	2.071	1.896
200	1.372	1.424	1.398	1.223
100	0.726	0.739	0.733	0.558
50	0.471	0.504	0.488	0.313
25	0.380	0.359	0.370	0.195
12.5	0.271	0.259	0.265	0.090
0	0.173	0.177	0.175

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q13526

Applications	ELISA
Research Area	PIN1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.