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Home / ELISA Kit

Human Protein patched homolog 1 (PTCH1) ELISA Kit

SKU : BHE13703291

PTCH1 encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis. This gene functions as a tumor suppressor.
Mutations of this gene have been associated with nevoid basal cell carcinoma syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.

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Mfr.No.	KTE61075
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein patched homolog 1 (PTCH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTCH1.
Alternative Names	PTCH1; RP11-435O5.3; BCNS; FLJ26746; FLJ42602; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11; OTTHUMP00000021709; OTTHUMP00000021710; PTCH protein +12b; PTCH protein +4 ; PTCH protein -10; patched
Detection Method	Colorimetric
Kit Components	Human Protein patched homolog 1 microplate Human Protein patched homolog 1 standard Human Protein patched homolog 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein patched homolog 1 (PTCH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTCH1. No significant cross-reactivity or interference between Human PTCH1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PTCH1 encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis. This gene functions as a tumor suppressor. Mutations of this gene have been associated with nevoid basal cell carcinoma syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.
Notes	This Human Protein patched homolog 1 (PTCH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTCH1 in samples. An antibody specific for PTCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTCH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5727

Applications	ELISA
Applications Notes	This Human Protein patched homolog 1 (PTCH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTCH1 in samples. An antibody specific for PTCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTCH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703291	KTE61075	48 T	$339		Add to Cart
BHE13703291	KTE61075	96 T	$589		Add to Cart
BHE13703291	KTE61075	96 Tx5	$2629		Add to Cart
BHE13703291	KTE61075	96 Tx50	$24569		Add to Cart

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