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Home / ELISA Kit

Human Protein-L-isoaspartate (PCMT1) ELISA Kit

SKU : BHE13703508

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues.
These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.

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Mfr.No.	KTE61292
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein-L-isoaspartate (PCMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCMT1.
Alternative Names	PCMT1; L-isoaspartyl/D-aspartyl methyltransferase; OTTHUMP00000017400; protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
Detection Method	Colorimetric
Kit Components	Human Protein-L-isoaspartate microplate Human Protein-L-isoaspartate standard Human Protein-L-isoaspartate detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein-L-isoaspartate (PCMT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCMT1. No significant cross-reactivity or interference between Human PCMT1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.
Notes	This Human Protein-L-isoaspartate (PCMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCMT1 in samples. An antibody specific for PCMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5110

Applications	ELISA
Applications Notes	This Human Protein-L-isoaspartate (PCMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCMT1 in samples. An antibody specific for PCMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703508	KTE61292	48 T	$339		Add to Cart
BHE13703508	KTE61292	96 T	$589		Add to Cart
BHE13703508	KTE61292	96 Tx5	$2629		Add to Cart
BHE13703508	KTE61292	96 Tx50	$24569		Add to Cart

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