Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

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Mfr.No.

CSB-E13343h

Alternative Names

Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II)

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.562	2.687	2.625	2.443
1000	1.839	1.865	1.852	1.670
500	1.104	1.135	1.120	0.938
250	0.643	0.638	0.641	0.459
125	0.412	0.435	0.424	0.242
62.5	0.321	0.341	0.331	0.149
31.25	0.262	0.248	0.255	0.073
0	0.181	0.183	0.182

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

Antigen	Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II)

Applications

ELISA

Research Area

PIVKA-II

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Diagnostic Performance of Alpha-Fetoprotein, Protein Induced by Vitamin K Absence, Osteopontin, Dickkopf-1 and Its Combinations for Hepatocellular Carcinoma Eun SunJang.et al,PLoS One.,2016 Mar17:14 Functional deficiency of vitamin K in hemodialysis patients in Upper Silesia in Poland Wyskida K.et al,Int Urol Nephrol.,2016 Mar 21. Biological Evidences of Hepatocellular Carcinoma Treatment with 1, 3, 4-oxadiazole-2-thiol as Anticancer Agent Mohamed S Gabry1.et al,Biomolecular Research & Therapeutics,/ ANNALS EXPRESS: Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis Unic A.et al,Ann Clin Biochem.,Aug 2

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BHE10503995	CSB-E13343h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.