Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13343h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II)

Recovery

The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PIVKA-II

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Diagnostic Performance of Alpha-Fetoprotein, Protein Induced by Vitamin K Absence, Osteopontin, Dickkopf-1 and Its Combinations for Hepatocellular Carcinoma Eun SunJang.et al,PLoS One.,2016 Mar17:14 Functional deficiency of vitamin K in hemodialysis patients in Upper Silesia in Poland Wyskida K.et al,Int Urol Nephrol.,2016 Mar 21. Biological Evidences of Hepatocellular Carcinoma Treatment with 1, 3, 4-oxadiazole-2-thiol as Anticancer Agent Mohamed S Gabry1.et al,Biomolecular Research & Therapeutics,/ ANNALS EXPRESS: Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis Unic A.et al,Ann Clin Biochem.,Aug 2

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