Human Protein-glutamine gamma-glutamyltransferase K(TGM1) ELISA kit

Human Protein-glutamine gamma-glutamyltransferase K(TGM1) ELISA kit

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Mfr.No.

CSB-EL023461HU

Alternative Names

transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase), ICR2, KTG, LI, LI1, TGASE, TGK, TGase K, epidermal TGase, transglutaminase 1, transglutaminase K, transglutaminase, keratinocyte

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ICOSLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-103
EDTA plasma (n=4)	94	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ICOSLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-94
1:2	Average %	96
	Range %	89-102
1:4	Average %	98
	Range %	95-106
1:5	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.748	2.789	2.769	2.592
3000	2.375	2.372	2.374	2.197
1500	1.834	1.816	1.825	1.648
750	1.061	1.047	1.054	0.877
375	0.632	0.636	0.634	0.457
187.5	0.427	0.441	0.434	0.257
93.75	0.259	0.269	0.264	0.087
0	0.179	0.174	0.177	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P22735
Antigen	transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)

Applications

ELISA

Research Area

TGM1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503992	CSB-EL023461HU	96T	$695	Add to Cart
BHE10503992	CSB-EL023461HU	5x96T	$3336	Add to Cart
BHE10503992	CSB-EL023461HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.