Human protein disulfide isomerase,PDI ELISA Kit

Human protein disulfide isomerase,PDI ELISA Kit

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Mfr.No.

CSB-E09003h

Alternative Names

prolyl 4-hydroxylase, beta polypeptide, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-106
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	85-96
1:2000	Average %	97
	Range %	91-104
1:4000	Average %	92
	Range %	85-100
1:8000	Average %	93
	Range %	83-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.248	2.205	2.227	2.072
100	1.196	1.266	1.231	1.076
50	0.675	0.663	0.669	0.514
25	0.472	0.453	0.463	0.308
12.5	0.335	0.314	0.325	0.170
6.25	0.221	0.207	0.214	0.059
3.12	0.200	0.194	0.197	0.042
0	0.155	0.154	0.155	?

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P07237
Antigen	prolyl 4-hydroxylase, beta polypeptide

Applications

ELISA

Research Area

P4HB

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503970	CSB-E09003h	96T	$790	Add to Cart
BHE10503970	CSB-E09003h	5x96T	$3792	Add to Cart
BHE10503970	CSB-E09003h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.