Human protein disulfide-isomerase A3,PDIA3 ELISA Kit

Human protein disulfide-isomerase A3,PDIA3 ELISA Kit

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Mfr.No.

CSB-E09798h

Sensitivity

0.31 ng/mL

Detection Range

1.25 ng/mL-80 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

protein disulfide isomerase family A, member 3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, 58 kDa microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58kDa, phospholipase C-alpha, protein disulfide isomerase

Recovery

The recovery of human Col ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Col ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-90
1:200	Average %	93
	Range %	88-98
1:400	Average %	94
	Range %	90-100
1:800	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.478	2.387	2.433	2.341
1000	1.811	1.752	1.782	1.690
500	1.237	1.142	1.190	1.098
250	0.863	0.813	0.838	0.746
125	0.398	0.374	0.386	0.294
62.5	0.211	0.198	0.205	0.113
31.25	0.145	0.141	0.143	0.051
0	0.093	0.090	0.092	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P30101

Applications	ELISA
Research Area	PDIA3

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503967	CSB-E09798h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.