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Home / ELISA Kit

Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit

SKU : BHE13703480

Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insulinoma subtraction cDNA library. The deduced 511-amino acid protein has a calculated molecular mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs . Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.

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Mfr.No.	KTE61264
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA2.
Alternative Names	PDIA2; LA16c-314G4.2; PDA2; PDI; PDIP; PDIR; Rho GDP dissociation inhibitor gamma; pancreatic protein disulfide isomerase; protein disulfide isomerase A2; protein disulfide isomerase; pancreatic; protein disul
Detection Method	Colorimetric
Kit Components	Human Protein disulfide-isomerase A2 microplate Human Protein disulfide-isomerase A2 standard Human Protein disulfide-isomerase A2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDIA2. No significant cross-reactivity or interference between Human PDIA2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insulinoma subtraction cDNA library. The deduced 511-amino acid protein has a calculated molecular mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs . Northe blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.
Notes	This Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA2 in samples. An antibody specific for PDIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	64714

Applications	ELISA
Applications Notes	This Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA2 in samples. An antibody specific for PDIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703480	KTE61264	48 T	$339		Add to Cart
BHE13703480	KTE61264	96 T	$589		Add to Cart
BHE13703480	KTE61264	96 Tx5	$2629		Add to Cart
BHE13703480	KTE61264	96 Tx50	$24569		Add to Cart

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