Human protein-arginine deiminase type-4(PADI4) ELISA kit

Human protein-arginine deiminase type-4(PADI4) ELISA kit

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Mfr.No.

CSB-E16219h

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peptidyl arginine deiminase, type IV, PAD, PAD4, PADI5, PDI4, PDI5, HL-60 PAD, PADI-H protein, peptidyl arginine deiminase, type V, protein-arginine deiminase

Recovery

The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	95
	Range %	88-102
1:4	Average %	94
	Range %	89-99
1:8	Average %	95
	Range %	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.557	2.458	2.508
2.5	1.495	1.481	1.488
10	1.053	1.045	1.049
40	0.650	0.636	0.643
125	0.408	0.392	0.400
500	0.222	0.214	0.218

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9UM07

Applications	ELISA
Research Area	PADI4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503953	CSB-E16219h	96T	$695	Add to Cart
BHE10503953	CSB-E16219h	5x96T	$3336	Add to Cart
BHE10503953	CSB-E16219h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.