Human Protein-arginine deiminase type-4 (PADI4)antibody ELISA Kit

Mfr.No.

CSB-E13109h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

Protein-arginine deiminase type-4 (PADI4)antibody, antibody for PAD, PAD4, PADI5, PDI4, PDI5, HL-60 PAD, PADI-H protein, peptidyl arginine deiminase, type V, protein-arginine deiminase

Recovery

The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-92
EDTA plasma (n=4)	92	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-105
1:2	Average %	102
	Range %	97-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	101
	Range %	97-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.684	2.799	2.742	2.633
5	2.178	2.349	2.264	2.155
2.5	1.444	1.489	1.467	1.358
1.25	0.783	0.795	0.789	0.680
0.625	0.376	0.392	0.384	0.275
0.312	0.258	0.264	0.261	0.152
0.156	0.201	0.208	0.205	0.096
0	0.108	0.109	0.109	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	PADI4 Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503952	CSB-E13109h	96T	$790	Add to Cart
BHE10503952	CSB-E13109h	5x96T	$3792	Add to Cart
BHE10503952	CSB-E13109h	10x96T	$7505	Add to Cart

Human Protein-arginine deiminase type-4 (PADI4)antibody ELISA Kit

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