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Home / ELISA Kit

Human Protein-arginine deiminase type-3 (PADI3) ELISA Kit

SKU : BHE13703536

Peptidyl arginine deiminase, type III, also known as PADI3, is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns.
The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes.Hair follicles, and epidermis at very low levels.

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Mfr.No.	KTE61320
Description	This Human Protein-arginine deiminase type-3 (PADI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI3.
Alternative Names	PADI3; hCG_25124; MGC126307; MGC126308; PAD3; PDI3; peptidylarginine deiminaseI; protein-arginine deiminase typeI
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Protein-arginine deiminase type-3 microplate Human Protein-arginine deiminase type-3 standard Human Protein-arginine deiminase type-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protein-arginine deiminase type-3 (PADI3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PADI3. No significant cross-reactivity or interference between Human PADI3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Peptidyl arginine deiminase, type III, also known as PADI3, is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes.Hair follicles, and epidermis at very low levels.
Notes	This Human Protein-arginine deiminase type-3 (PADI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI3 in samples. An antibody specific for PADI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	51702
Antigen	PADI3
Tag	PADI3
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Protein-arginine deiminase type-3 (PADI3) ELISA Kit employs a two-site sandwich ELISA to quantitate PADI3 in samples. An antibody specific for PADI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPADI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703536	KTE61320	48 T	$339		Add to Cart
BHE13703536	KTE61320	96 T	$589		Add to Cart
BHE13703536	KTE61320	96 Tx5	$2629		Add to Cart
BHE13703536	KTE61320	96 Tx50	$24569		Add to Cart

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