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Home / ELISA Kit

Human Proteasome subunit alpha type-7 (PSMA7) ELISA Kit

SKU : BHE13703300

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMA7 has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication.
This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.

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Mfr.No.	KTE61084
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proteasome subunit alpha type-7 (PSMA7) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA7.
Alternative Names	PSMA7; RP5-1005F21.4; C6; HSPC; MGC3755; RC6-1; XAPC7; proteasome alpha 7 subunit; proteasome subunit RC6-1; proteasome subunit XAPC7; proteasome subunit alpha 4
Detection Method	Colorimetric
Kit Components	Human Proteasome subunit alpha type-7 microplate Human Proteasome subunit alpha type-7 standard Human Proteasome subunit alpha type-7 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proteasome subunit alpha type-7 (PSMA7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMA7. No significant cross-reactivity or interference between Human PSMA7 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMA7 has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C inteal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alteative splicing may exist but alteative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9.
Notes	This Human Proteasome subunit alpha type-7 (PSMA7) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA7 in samples. An antibody specific for PSMA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5688

Applications	ELISA
Applications Notes	This Human Proteasome subunit alpha type-7 (PSMA7) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA7 in samples. An antibody specific for PSMA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703300	KTE61084	48 T	$339		Add to Cart
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BHE13703300	KTE61084	96 Tx50	$24569		Add to Cart

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