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Home / ELISA Kit

Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit

SKU : BHE13703301

An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.The cDNA encoded a 241-amino acid polypeptide. The calculated and observed molecular masses were 26 kD and 29 kD, respectively. Northern blot analysis revealed an mRNA of 1.2 kb in both human placenta and HeLa cells.The alpha subunits comprise the outer rings of the proteasome. Some alpha subunits contain a functional nuclear localization signal; proteasomes are found in both the nuclear and cytoplasmic compartments of the cell. Alpha subunits may constitute a physical barrier that limits access of cytosolic proteins into the inner proteolytic chamber.

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Mfr.No.	KTE61085
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA5.
Alternative Names	PSMA5; MGC117302; MGC125802; MGC125803; MGC125804; PSC5; ZETA; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome alpha 5 subunit; proteasome component 5;
Detection Method	Colorimetric
Kit Components	Human Proteasome subunit alpha type-5 microplate Human Proteasome subunit alpha type-5 standard Human Proteasome subunit alpha type-5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMA5. No significant cross-reactivity or interference between Human PSMA5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.The cDNA encoded a 241-amino acid polypeptide. The calculated and observed molecular masses were 26 kD and 29 kD, respectively. Northern blot analysis revealed an mRNA of 1.2 kb in both human placenta and HeLa cells.The alpha subunits comprise the outer rings of the proteasome. Some alpha subunits contain a functional nuclear localization signal; proteasomes are found in both the nuclear and cytoplasmic compartments of the cell. Alpha subunits may constitute a physical barrier that limits access of cytosolic proteins into the inner proteolytic chamber.
Notes	This Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA5 in samples. An antibody specific for PSMA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5686

Applications	ELISA
Applications Notes	This Human Proteasome subunit alpha type-5 (PSMA5) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA5 in samples. An antibody specific for PSMA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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