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Home / ELISA Kit

Human Proteasome subunit alpha type-1 (PSMA1) ELISA Kit

SKU : BHE13703302

Proteasome subunit alpha type-1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.
Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.

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Mfr.No.	KTE61086
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proteasome subunit alpha type-1 (PSMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA1.
Alternative Names	PSMA1; HC2; MGC14542; MGC14575; MGC14751; MGC1667; MGC21459; MGC22853; MGC23915; NU; PROS30; 30 kDa prosomal protein; macropain subunit nu; multicatalytic endopeptidase complex subunit C2; proteasome alpha 1
Detection Method	Colorimetric
Kit Components	Human Proteasome subunit alpha type-1 microplate Human Proteasome subunit alpha type-1 standard Human Proteasome subunit alpha type-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proteasome subunit alpha type-1 (PSMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMA1. No significant cross-reactivity or interference between Human PSMA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Proteasome subunit alpha type-1 is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.
Notes	This Human Proteasome subunit alpha type-1 (PSMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA1 in samples. An antibody specific for PSMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5682

Applications	ELISA
Applications Notes	This Human Proteasome subunit alpha type-1 (PSMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMA1 in samples. An antibody specific for PSMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703302	KTE61086	48 T	$339		Add to Cart
BHE13703302	KTE61086	96 T	$589		Add to Cart
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BHE13703302	KTE61086	96 Tx50	$24569		Add to Cart

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