Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

Human proteasome (prosome, macropain) subunit, beta type, 2 (PSMB2) ELISA kit

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Mfr.No.

CSB-E17836h

Alternative Names

proteasome (prosome, macropain) subunit, beta type, 2, RP5-983H21.1, HC7-I, MGC104215, MGC126885, macropain subunit C7-I, multicatalytic endopeptidase complex subunit C7-1, proteasome beta 2 subunit, proteasome component C7-I, proteasome subunit, beta type,

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	99	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-93
1:2	Average %	104
	Range %	98-107
1:4	Average %	96
	Range %	88-99
1:8	Average %	96
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.551	2.487	2.519	2.354
5	2.164	2.112	2.138	1.973
2.5	1.651	1.688	1.670	1.505
1.25	1.043	1.032	1.038	0.873
0.625	0.660	0.645	0.653	0.488
0.312	0.474	0.467	0.471	0.306
0.156	0.268	0.272	0.270	0.105
0	0.163	0.167	0.165

Species

Homo sapiens

Sensitivity

6.2 pg/mL

Limit Of Detection

450 nm

UniProt ID	P49721
Antigen	proteasome (prosome, macropain) subunit, beta type, 2

Applications

ELISA

Research Area

PSMB2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503943	CSB-E17836h	96T	$790	Add to Cart
BHE10503943	CSB-E17836h	5x96T	$3792	Add to Cart
BHE10503943	CSB-E17836h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.