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Home / ELISA Kit

Human Protamine-1 (PRM1) ELISA Kit

SKU : BHE13703357

Protamines are small, arginine-rich, nuclear proteins that replace histones late in the haploid phase of spermatogenesis and are believed essential for sperm head condensation and DNA stabilization. Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine. The 2 human protamines are denoted P1 and P2 (PRM2).
PRM1 and PRM2 have a single intron, consisting of 91 and 163 bp, respectively. Both genes were found to contain typical TATAA and CAAT boxes at conventional distances from the transcription start points which, by use of primary extension experiments, were assigned to nucleotides -91 and -110 for PRM1 and PRM2, respectively.

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Mfr.No.	KTE61141
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protamine-1 (PRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM1.
Alternative Names	PRM1; CT94.1; P1; cancer/testis antigen family 94; member 1; sperm protamine P1; testis specific protamine 1
Detection Method	Colorimetric
Kit Components	Human Protamine-1 microplate Human Protamine-1 standard Human Protamine-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protamine-1 (PRM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRM1. No significant cross-reactivity or interference between Human PRM1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protamines are small, arginine-rich, nuclear proteins that replace histones late in the haploid phase of spermatogenesis and are believed essential for sperm head condensation and DNA stabilization. Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine. The 2 human protamines are denoted P1 and P2 (PRM2). PRM1 and PRM2 have a single intron, consisting of 91 and 163 bp, respectively. Both genes were found to contain typical TATAA and CAAT boxes at conventional distances from the transcription start points which, by use of primary extension experiments, were assigned to nucleotides -91 and -110 for PRM1 and PRM2, respectively.
Notes	This Human Protamine-1 (PRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM1 in samples. An antibody specific for PRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5619

Applications	ELISA
Applications Notes	This Human Protamine-1 (PRM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM1 in samples. An antibody specific for PRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703357	KTE61141	48 T	$359		Add to Cart
BHE13703357	KTE61141	96 T	$619		Add to Cart
BHE13703357	KTE61141	96 Tx5	$2809		Add to Cart
BHE13703357	KTE61141	96 Tx50	$26209		Add to Cart

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