Human Protamine 1,PRM1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09630h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

protamine 1, CT94.1, P1, cancer/testis antigen family 94, member 1, sperm protamine P1, testis specific protamine 1

Recovery

The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	98	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-90
1:200	Average %	98
	Range %	90-105
1:400	Average %	95
	Range %	90-100
1:800	Average %	92
	Range %	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04553

Applications	ELISA
Research Area	PRM1

Storage	Store at 2-8°C. Please refer to protocol.

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