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Home / ELISA Kit

Human Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit

SKU : BHE13703274

PTOV1 was predicted to form alternating alpha helices and beta sheets. Normal prostate expressed low PTOV1 levels. Western blot analysis of prostate tumor tissue detected PTOV1 at an apparent molecular mass of 58 kD. Immunocytochemical analysis detected endogenous PTOV1 in the cytoplasm, concentrated around the nucleus.PTOV1 was overexpressed in 71% of 38 prostate carcinomas and in 80% of samples with prostate intraepithelial neoplasia. High levels of PTOV1 in tumors correlated significantly with proliferative index and localization of PTOV1 in the nucleus. In quiescent cultured prostate tumor cells, PTOV1 localized to the cytoplasm and was excluded from nuclei. After serum stimulation, PTOV1 partially translocated to the nucleus at the beginning of S phase.

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Mfr.No.	KTE61058
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTOV1.
Alternative Names	PTOV1; ACID2; DKFZp586I111; MGC71475; prostate tumor overexpressed gene 1
Detection Method	Colorimetric
Kit Components	Human Prostate tumor-overexpressed gene 1 protein microplate Human Prostate tumor-overexpressed gene 1 protein standard Human Prostate tumor-overexpressed gene 1 protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTOV1. No significant cross-reactivity or interference between Human PTOV1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PTOV1 was predicted to form alternating alpha helices and beta sheets. Normal prostate expressed low PTOV1 levels. Western blot analysis of prostate tumor tissue detected PTOV1 at an apparent molecular mass of 58 kD. Immunocytochemical analysis detected endogenous PTOV1 in the cytoplasm, concentrated around the nucleus.PTOV1 was overexpressed in 71% of 38 prostate carcinomas and in 80% of samples with prostate intraepithelial neoplasia. High levels of PTOV1 in tumors correlated significantly with proliferative index and localization of PTOV1 in the nucleus. In quiescent cultured prostate tumor cells, PTOV1 localized to the cytoplasm and was excluded from nuclei. After serum stimulation, PTOV1 partially translocated to the nucleus at the beginning of S phase.
Notes	This Human Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTOV1 in samples. An antibody specific for PTOV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTOV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTOV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTOV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	53635

Applications	ELISA
Applications Notes	This Human Prostate tumor-overexpressed gene 1 protein (PTOV1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTOV1 in samples. An antibody specific for PTOV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTOV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTOV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTOV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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