Human Prostasin - PRSS8 ELISA Kit

Mfr.No.

E5944Hu

Alternative Names

CAP1;Channel-activating protease 1;Prostasin;Prostasin heavy chain;Prostasin light chain;PRSS 8;PRSS8;Serine protease 8

Reactivity

Human

Detection Range

0.5-300ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36431

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.28ng/mL

Background

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium Channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).

Gene ID	5652
UniProt ID	Q16651
Antigen	PRSS8

Research Area

Cell Biology

Protocol

These standard curves of E5944Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/mL	2.648	2.574	2.519
	2.5
80ng/mL	1.41	1.416	1.361
	1.422
40ng/mL	0.883	0.836	0.781
	0.79
20ng/mL	0.381	0.418	0.363
	0.454
10ng/mL	0.228	0.229	0.174
	0.23
0ng/mL	0.044	0.055	0
	0.066

Precision

We measured random samples of E5944Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	54.9	2.58	4.7
Intra-Assay	2	18	88.2	2.65	3
Intra-Assay	3	18	36.4	1.68	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107306	E5944Hu	48T	$320		Add to Cart
BHE12107306	E5944Hu	96T	$458		Add to Cart

Human Prostasin - PRSS8 ELISA Kit

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