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Home / ELISA Kit

Human Prostaglandin reductase 1 (PTGR1) ELISA Kit

SKU : BHE13703284

PTGR1 isan enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants.
Leukotriene B(4) 12-hydroxydehydrogenase catalyzes the conversion of leukotriene B(4) into 12-oxo-leukotriene B(4), its less-active metabolite.The identity between the porcine and human sequences they obtained is 83.5% at the amino acid level and 84.7% at the nucleotide level. Both enzymes are highly homologous with a protein, AdRab-F, expressed only in adult rabbit small intestine

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Mfr.No.	KTE61068
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prostaglandin reductase 1 (PTGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGR1.
Alternative Names	PTGR1; RP11-16L21.1; FLJ99229; LTB4DH; MGC34943; PGR1; ZADH3; 15-oxoprostaglandin 13-reductase; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; leukotriene B4 12-hydroxydehydrogenase; zinc binding alco
Detection Method	Colorimetric
Kit Components	Human Prostaglandin reductase 1 microplate Human Prostaglandin reductase 1 standard Human Prostaglandin reductase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prostaglandin reductase 1 (PTGR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTGR1. No significant cross-reactivity or interference between Human PTGR1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PTGR1 isan enzyme that is involved in the inactivation of the chemotactic factor, leukotriene B4. The encoded protein specifically catalyzes the NADP+ dependent conversion of leukotriene B4 to 12-oxo-leukotriene B4. A pseudogene of this gene is found on chromosome 1. Alternative splicing results in multiple transcript variants. Leukotriene B(4) 12-hydroxydehydrogenase catalyzes the conversion of leukotriene B(4) into 12-oxo-leukotriene B(4), its less-active metabolite.The identity between the porcine and human sequences they obtained is 83.5% at the amino acid level and 84.7% at the nucleotide level. Both enzymes are highly homologous with a protein, AdRab-F, expressed only in adult rabbit small intestine
Notes	This Human Prostaglandin reductase 1 (PTGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGR1 in samples. An antibody specific for PTGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	22949

Applications	ELISA
Applications Notes	This Human Prostaglandin reductase 1 (PTGR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGR1 in samples. An antibody specific for PTGR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703284	KTE61068	48 T	$339		Add to Cart
BHE13703284	KTE61068	96 T	$589		Add to Cart
BHE13703284	KTE61068	96 Tx5	$2629		Add to Cart
BHE13703284	KTE61068	96 Tx50	$24569		Add to Cart

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