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Home / ELISA Kit

Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit

SKU : BHE13704746

Prostaglandin D synthase catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2).Prostaglandin D synthase induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGD synthase have been identified, namely the lipocalin type enzyme (b-trace) and the hematopoietic enzyme. Lipocalin type prostaglandin D synthase is localized in the central nervous system and male genital organs of various mammals and the human heart. This enzyme has been identified as b-trace, which is a major protein in human cerebrospinal fluid. Hematopoietic prostaglandin D synthase is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes.

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Mfr.No.	KTE62539
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGDS.
Alternative Names	PTGDS; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla
Detection Method	Colorimetric
Kit Components	Human Prostaglandin-H2 D-isomerase microplate Human Prostaglandin-H2 D-isomerase standard Human Prostaglandin-H2 D-isomerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTGDS. No significant cross-reactivity or interference between Human PTGDS and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prostaglandin D synthase catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2).Prostaglandin D synthase induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGD synthase have been identified, namely the lipocalin type enzyme (b-trace) and the hematopoietic enzyme. Lipocalin type prostaglandin D synthase is localized in the central nervous system and male genital organs of various mammals and the human heart. This enzyme has been identified as b-trace, which is a major protein in human cerebrospinal fluid. Hematopoietic prostaglandin D synthase is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes.
Notes	This Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGDS in samples. An antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGDS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGDS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5730

Applications	ELISA
Applications Notes	This Human Prostaglandin-H2 D-isomerase (PTGDS) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGDS in samples. An antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGDS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGDS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704746	KTE62539	48 T	$359		Add to Cart
BHE13704746	KTE62539	96 T	$619		Add to Cart
BHE13704746	KTE62539	96 Tx5	$2809		Add to Cart
BHE13704746	KTE62539	96 Tx50	$26209		Add to Cart

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