Human prostacycline,PGI2 ELISA Kit

Human prostacycline,PGI2 ELISA Kit

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Mfr.No.

CSB-E09591h

Alternative Names

prostacycline,PGI2

Reactivity

Human

Detection Range

50 pg/mL-2000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat Col ? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	85-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col ? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	98
	Range %	92-104
1:4	Average %	95
	Range %	92-98
1:8	Average %	86
	Range %	80-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.585	2.532	2.559	2.415
5	1.795	1.719	1.757	1.613
2.5	1.083	1.021	1.052	0.908
1.25	0.657	0.705	0.681	0.537
0.625	0.418	0.450	0.434	0.290
0.312	0.299	0.314	0.307	0.163
0.156	0.242	0.229	0.236	0.092
0	0.146	0.141	0.144	?

Species

Homo sapiens

Sensitivity

50 pg/mL

Limit Of Detection

450 nm

Antigen	prostacycline,PGI2

Applications

ELISA

Research Area

PGI2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A new approach to an old hypothesis; phototherapy does not affect ductal patency via PGE2 and PGI2 Surmeli-Onay O et al,J Matern Fetal Neonatal Med,2014 Mar 4. Donor-dependent variation of human umbilical cord blood mesenchymal stem cells in response to hypoxic preconditioning and amelioration of limb ischemia Insung Kang.et al,Experimental & Molecular Medicinevolume,20 April

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SKU	Supplier No.	Size	Your price
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BHE10503925	CSB-E09591h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.