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Home / ELISA Kit

Human Prosaposin (PSAP) ELISA Kit

SKU : BHE13703310

Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO(S)teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases.

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Mfr.No.	KTE61094
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP.
Alternative Names	PSAP; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1
Detection Method	Colorimetric
Kit Components	Human Prosaposin microplate Human Prosaposin standard Human Prosaposin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prosaposin (PSAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSAP. No significant cross-reactivity or interference between Human PSAP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO(S)teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases.
Notes	This Human Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP in samples. An antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5660

Applications	ELISA
Applications Notes	This Human Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP in samples. An antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703310	KTE61094	48 T	$359		Add to Cart
BHE13703310	KTE61094	96 T	$619		Add to Cart
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BHE13703310	KTE61094	96 Tx50	$26209		Add to Cart

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