Human Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

Human Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

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Mfr.No.

CSB-EL017647HU

Alternative Names

proprotein convertase subtilisin/kexin type 9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, convertase subtilisin/kexin type 9 preproprotein, neural apoptosis regulated convertase 1

Reactivity

Human

Detection Range

0.45 ng/mL-30 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse SPARCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	95	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SPARCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	95
	Range %	90-101
1:4	Average %	94
	Range %	90-97
1:8	Average %	97
	Range %	95-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.588	2.542	2.565	2.416
2500	1.788	1.745	1.767	1.618
1250	1.079	1.066	1.073	0.924
625	0.698	0.701	0.700	0.551
312.5	0.443	0.434	0.439	0.290
156	0.298	0.278	0.288	0.139
78	0.222	0.230	0.226	0.077
0	0.150	0.148	0.149	?

Species

Homo sapiens

Sensitivity

0.11 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q8NBP7
Antigen	Proprotein Convertase Subtilisin/Kexin Type 9

Applications

ELISA

Research Area

PCSK9

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503920	CSB-EL017647HU	96T	$595	Add to Cart
BHE10503920	CSB-EL017647HU	5x96T	$2856	Add to Cart
BHE10503920	CSB-EL017647HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.