Human Prolyl Endopeptidase

Mfr.No.

E3950Hu

Sensitivity

0.02ng/mL

Detection Range

0.05-30ng/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Prolyl Endopeptidase (also known as PREP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

PE;Post-proline cleaving enzyme;PREP;Prolyl endopeptidase

Molecular Weight

80700

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3950Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.1	0.24	4.6
Intra-Assay	2	18	12.1	0.45	3.7
Intra-Assay	3	18	4	0.24	5.9

Background

Cleaves Peptide bonds on the C-terminal side of prolyl residues within Peptides that are up to approximately 30 amino acids long.

Protocol

These standard curves of E3950Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/mL	2.269	2.286	2.244
	2.303
10ng/mL	1.378	1.377	1.335
	1.376
5ng/mL	0.666	0.647	0.605
	0.628
2.5ng/mL	0.529	0.506	0.464
	0.483
1.25ng/mL	0.219	0.228	0.186
	0.236
0ng/mL	0.055	0.042	0
	0.029

Gene ID	5550
UniProt ID	P48147
Antigen	PREP

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105384	E3950Hu	48T	$320		Add to Cart
BHE12105384	E3950Hu	96T	$458		Add to Cart

Human Prolyl Endopeptidase - PREP ELISA Kit

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