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Home / ELISA Kit

Human Prolyl endopeptidase-like (PREPL) ELISA Kit

SKU : BHE13703387

PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene.
The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.

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Mfr.No.	KTE61171
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prolyl endopeptidase-like (PREPL) ELISA Kit employs a two-site sandwich ELISA to quantitate PREPL.
Alternative Names	PREPL; FLJ16627; KIAA0436; OTTHUMP00000202420; OTTHUMP00000202421; OTTHUMP00000202424; OTTHUMP00000202425; OTTHUMP00000202426; putative prolyl oligopeptidase
Detection Method	Colorimetric
Kit Components	Human Prolyl endopeptidase-like microplate Human Prolyl endopeptidase-like standard Human Prolyl endopeptidase-like detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prolyl endopeptidase-like (PREPL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PREPL. No significant cross-reactivity or interference between Human PREPL and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.
Notes	This Human Prolyl endopeptidase-like (PREPL) ELISA Kit employs a two-site sandwich ELISA to quantitate PREPL in samples. An antibody specific for PREPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9581

Applications	ELISA
Applications Notes	This Human Prolyl endopeptidase-like (PREPL) ELISA Kit employs a two-site sandwich ELISA to quantitate PREPL in samples. An antibody specific for PREPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703387	KTE61171	48 T	$339		Add to Cart
BHE13703387	KTE61171	96 T	$589		Add to Cart
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