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Home / ELISA Kit

Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit

SKU : BHE13703542

P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525 amino acids and shares 91% and 94% amino acid identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1.

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Mfr.No.	KTE61326
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA3.
Alternative Names	P4HA3; C-P4H alphaI; collagen prolyl 4-hydroxylase alpha(III); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypeptideI; prolyl 4-hydroxylase; alphaI subunit
Detection Method	Colorimetric
Kit Components	Human Prolyl 4-hydroxylase subunit alpha-3 microplate Human Prolyl 4-hydroxylase subunit alpha-3 standard Human Prolyl 4-hydroxylase subunit alpha-3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HA3. No significant cross-reactivity or interference between Human P4HA3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525 amino acids and shares 91% and 94% amino acid identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1.
Notes	This Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA3 in samples. An antibody specific for P4HA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	283208

Applications	ELISA
Applications Notes	This Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA3 in samples. An antibody specific for P4HA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703542	KTE61326	48 T	$339		Add to Cart
BHE13703542	KTE61326	96 T	$589		Add to Cart
BHE13703542	KTE61326	96 Tx5	$2629		Add to Cart
BHE13703542	KTE61326	96 Tx50	$24569		Add to Cart

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