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Home / ELISA Kit

Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit

SKU : BHE13703543

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. The beta subunit (P4HB) is an unusual multifunctional polypeptide identical to protein disulfide isomerase. P4HA2 is one of at least 2 alpha subunit isoforms. This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.

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Mfr.No.	KTE61327
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2.
Alternative Names	P4HA2; 4-PH alpha 2; C-P4Halpha(II); OTTHUMP00000065966; OTTHUMP00000065969; collagen prolyl 4-hydroxylase alpha(II); procollagen-proline; 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase); alpha polypepti
Detection Method	Colorimetric
Kit Components	Human Prolyl 4-hydroxylase subunit alpha-2 microplate Human Prolyl 4-hydroxylase subunit alpha-2 standard Human Prolyl 4-hydroxylase subunit alpha-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HA2. No significant cross-reactivity or interference between Human P4HA2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. The beta subunit (P4HB) is an unusual multifunctional polypeptide identical to protein disulfide isomerase. P4HA2 is one of at least 2 alpha subunit isoforms. This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.
Notes	This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2 in samples. An antibody specific for P4HA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8974

Applications	ELISA
Applications Notes	This Human Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) ELISA Kit employs a two-site sandwich ELISA to quantitate P4HA2 in samples. An antibody specific for P4HA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP4HA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P4HA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P4HA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703543	KTE61327	48 T	$339		Add to Cart
BHE13703543	KTE61327	96 T	$589		Add to Cart
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