Human Prolyl 3-hydroxylase 1

Mfr.No.

E7297Hu

Alternative Names

Growth suppressor 1;Leprecan-1;Leucine- and proline-enriched proteoglycan 1;P3H1;Prolyl 3-hydroxylase 1

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

83394

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.011ng/ml

Background

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.

Gene ID	64175
UniProt ID	Q32P28
Antigen	P3H1

Research Area

Signal Transduction

Protocol

These standard curves of E7297Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.046	1.955	1.908
	1.864
3.2ng/ml	1.107	1.078	1.031
	1.049
1.6ng/ml	0.51	0.507	0.46
	0.503
0.8ng/ml	0.174	0.214	0.168
	0.254
0.4ng/ml	0.166	0.146	0.099
	0.125
0ng/ml	0.036	0.047	0
	0.057

Precision

We measured random samples of E7297Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.3	0.17	3.2
Intra-Assay	2	18	2.4	0.1	4
Intra-Assay	3	18	1.7	0.08	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115523	E7297Hu	48T	$320		Add to Cart
BHE12115523	E7297Hu	96T	$458		Add to Cart

Human Prolyl 3-hydroxylase 1 - P3H1 ELISA Kit

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