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Home / ELISA Kit

Human Proline/serine-rich coiled-coil protein 1 (PSRC1) ELISA Kit

SKU : BHE13703294

PSRC1 encodes a proline-rich protein. Studies of the related mouse gene suggest that this gene is regulated by p53 and may participate in p53-mediated growth suppression. Alternatively spliced transcript variants encoding different isoforms have been described.
The deduced 333-amino acid protein shares 68.2% identity with mouse Dda3, with highest conservation in the N-terminal half. Both mouse and human DDA3 are rich in serine and proline and contain a coiled-coil domain, and 3 of 6 PxxP motifs in mouse Dda3 are conserved in human DDA3. PCR analysis revealed variable DDA3 expression in all human tissues examined except skeletal muscle. Highest expression was detected in fetal thymus and adult brain.

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Mfr.No.	KTE61078
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proline/serine-rich coiled-coil protein 1 (PSRC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSRC1.
Alternative Names	PSRC1; RP11-297O4.2; DDA3; FP3214; MGC1780; OTTHUMP00000205834; OTTHUMP00000205835; OTTHUMP00000205836; differential display and activated by p53; p53-regulated DDA3
Detection Method	Colorimetric
Kit Components	Human Proline/serine-rich coiled-coil protein 1 microplate Human Proline/serine-rich coiled-coil protein 1 standard Human Proline/serine-rich coiled-coil protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proline/serine-rich coiled-coil protein 1 (PSRC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSRC1. No significant cross-reactivity or interference between Human PSRC1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PSRC1 encodes a proline-rich protein. Studies of the related mouse gene suggest that this gene is regulated by p53 and may participate in p53-mediated growth suppression. Alteatively spliced transcript variants encoding different isoforms have been described. The deduced 333-amino acid protein shares 68.2% identity with mouse Dda3, with highest conservation in the N-terminal half. Both mouse and human DDA3 are rich in serine and proline and contain a coiled-coil domain, and 3 of 6 PxxP motifs in mouse Dda3 are conserved in human DDA3. PCR analysis revealed variable DDA3 expression in all human tissues examined except skeletal muscle. Highest expression was detected in fetal thymus and adult brain.
Notes	This Human Proline/serine-rich coiled-coil protein 1 (PSRC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSRC1 in samples. An antibody specific for PSRC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSRC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSRC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSRC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	84722

Applications	ELISA
Applications Notes	This Human Proline/serine-rich coiled-coil protein 1 (PSRC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PSRC1 in samples. An antibody specific for PSRC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSRC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSRC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSRC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703294	KTE61078	48 T	$339		Add to Cart
BHE13703294	KTE61078	96 T	$589		Add to Cart
BHE13703294	KTE61078	96 Tx5	$2629		Add to Cart
BHE13703294	KTE61078	96 Tx50	$24569		Add to Cart

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