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Home / ELISA Kit

Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit

SKU : BHE13703372

As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. collagen Q (ColQ) anchors AChE at the neuromuscular junction.PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain.
PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. AChE is actually anchored in neural cell membranes through its interaction with PRiMA. PRiMA anchors AChE in mammalian brain and muscle cell membranes. Required to anchor acetylcholinesteras

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Mfr.No.	KTE61156
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIMA1.
Alternative Names	PRIMA1; PRIMA; OTTHUMP00000180666; acetylcholinesterase membrane anchor precursor PRiMA
Detection Method	Colorimetric
Kit Components	Human Proline-rich membrane anchor 1 microplate Human Proline-rich membrane anchor 1 standard Human Proline-rich membrane anchor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRIMA1. No significant cross-reactivity or interference between Human PRIMA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. collagen Q (ColQ) anchors AChE at the neuromuscular junction.PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain. PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. AChE is actually anchored in neural cell membranes through its interaction with PRiMA. PRiMA anchors AChE in mammalian brain and muscle cell membranes. Required to anchor acetylcholinesteras
Notes	This Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIMA1 in samples. An antibody specific for PRIMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	145270

Applications	ELISA
Applications Notes	This Human Proline-rich membrane anchor 1 (PRIMA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRIMA1 in samples. An antibody specific for PRIMA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRIMA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRIMA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRIMA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703372	KTE61156	48 T	$339		Add to Cart
BHE13703372	KTE61156	96 T	$589		Add to Cart
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BHE13703372	KTE61156	96 Tx50	$24569		Add to Cart

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