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Home / ELISA Kit

Human Prolactin regulatory element-binding protein (PREB) ELISA Kit

SKU : BHE13703393

Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene.This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.

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Mfr.No.	KTE61177
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prolactin regulatory element-binding protein (PREB) ELISA Kit employs a two-site sandwich ELISA to quantitate PREB.
Alternative Names	PREB; MGC3467; SEC12; prolactin regulatory binding-element protein; prolactin regulatory element binding protein
Detection Method	Colorimetric
Kit Components	Human Prolactin regulatory element-binding protein microplate Human Prolactin regulatory element-binding protein standard Human Prolactin regulatory element-binding protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prolactin regulatory element-binding protein (PREB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PREB. No significant cross-reactivity or interference between Human PREB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene.This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.
Notes	This Human Prolactin regulatory element-binding protein (PREB) ELISA Kit employs a two-site sandwich ELISA to quantitate PREB in samples. An antibody specific for PREB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10113

Applications	ELISA
Applications Notes	This Human Prolactin regulatory element-binding protein (PREB) ELISA Kit employs a two-site sandwich ELISA to quantitate PREB in samples. An antibody specific for PREB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPREB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PREB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PREB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703393	KTE61177	48 T	$339		Add to Cart
BHE13703393	KTE61177	96 T	$589		Add to Cart
BHE13703393	KTE61177	96 Tx5	$2629		Add to Cart
BHE13703393	KTE61177	96 Tx50	$24569		Add to Cart

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